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NPC-ABS/FDS mediated deep chemotherapy and induced immune activation. ( A , B ) Confocal microscopy images in 3D tumor spheres after treated with different groups (a. NPC-FCS, b. NPC-FCS+NPC-AEAA-SLN, c. NPC-FCS+BAE-Sol, d. NPC-FCS+ABS, and e. NPC-ABS/FCS. Z-axis scan spacing = 10 μm. BAE = 2 μg/mL in A and BAE = 5 μg/mL in B, respectively; the magnification was 10×). ( C ) Representative photomicrographs of tumor biopsies treated with different groups (a. normal saline, b. NPC-AEAA-SLN, c. BAE-SLN, d. ABS, and e. NPC-ABS). Tumor blood vessels were labeled with <t>FITC-CD31;</t> the magnification was 20×). Relative expression quantity of immune-associated cells in mouse tumors after administration of different preparations of ( D ) CD4 + T cells, ( E ) CD8 + T cells, and ( F ) Tregs (a. normal saline, b. NPC-AEAA-SLN, c. BAE-SLN, d. ABS, and e. NPC-ABS. Mean ± SD, n = 3. ** p < 0.01, *** p < 0.001. Blue color represented positive cells; the magnification was 10×).
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NPC-ABS/FDS mediated deep chemotherapy and induced immune activation. ( A , B ) Confocal microscopy images in 3D tumor spheres after treated with different groups (a. NPC-FCS, b. NPC-FCS+NPC-AEAA-SLN, c. NPC-FCS+BAE-Sol, d. NPC-FCS+ABS, and e. NPC-ABS/FCS. Z-axis scan spacing = 10 μm. BAE = 2 μg/mL in A and BAE = 5 μg/mL in B, respectively; the magnification was 10×). ( C ) Representative photomicrographs of tumor biopsies treated with different groups (a. normal saline, b. NPC-AEAA-SLN, c. BAE-SLN, d. ABS, and e. NPC-ABS). Tumor blood vessels were labeled with <t>FITC-CD31;</t> the magnification was 20×). Relative expression quantity of immune-associated cells in mouse tumors after administration of different preparations of ( D ) CD4 + T cells, ( E ) CD8 + T cells, and ( F ) Tregs (a. normal saline, b. NPC-AEAA-SLN, c. BAE-SLN, d. ABS, and e. NPC-ABS. Mean ± SD, n = 3. ** p < 0.01, *** p < 0.001. Blue color represented positive cells; the magnification was 10×).
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NPC-ABS/FDS mediated deep chemotherapy and induced immune activation. ( A , B ) Confocal microscopy images in 3D tumor spheres after treated with different groups (a. NPC-FCS, b. NPC-FCS+NPC-AEAA-SLN, c. NPC-FCS+BAE-Sol, d. NPC-FCS+ABS, and e. NPC-ABS/FCS. Z-axis scan spacing = 10 μm. BAE = 2 μg/mL in A and BAE = 5 μg/mL in B, respectively; the magnification was 10×). ( C ) Representative photomicrographs of tumor biopsies treated with different groups (a. normal saline, b. NPC-AEAA-SLN, c. BAE-SLN, d. ABS, and e. NPC-ABS). Tumor blood vessels were labeled with FITC-CD31; the magnification was 20×). Relative expression quantity of immune-associated cells in mouse tumors after administration of different preparations of ( D ) CD4 + T cells, ( E ) CD8 + T cells, and ( F ) Tregs (a. normal saline, b. NPC-AEAA-SLN, c. BAE-SLN, d. ABS, and e. NPC-ABS. Mean ± SD, n = 3. ** p < 0.01, *** p < 0.001. Blue color represented positive cells; the magnification was 10×).

Journal: Pharmaceutics

Article Title: Taming the Tumor Stroma: A Two-Stage Targeted Nanocapsule for Potent Deep Chemo-Immunotherapy in Triple-Negative Breast Cancer

doi: 10.3390/pharmaceutics18020184

Figure Lengend Snippet: NPC-ABS/FDS mediated deep chemotherapy and induced immune activation. ( A , B ) Confocal microscopy images in 3D tumor spheres after treated with different groups (a. NPC-FCS, b. NPC-FCS+NPC-AEAA-SLN, c. NPC-FCS+BAE-Sol, d. NPC-FCS+ABS, and e. NPC-ABS/FCS. Z-axis scan spacing = 10 μm. BAE = 2 μg/mL in A and BAE = 5 μg/mL in B, respectively; the magnification was 10×). ( C ) Representative photomicrographs of tumor biopsies treated with different groups (a. normal saline, b. NPC-AEAA-SLN, c. BAE-SLN, d. ABS, and e. NPC-ABS). Tumor blood vessels were labeled with FITC-CD31; the magnification was 20×). Relative expression quantity of immune-associated cells in mouse tumors after administration of different preparations of ( D ) CD4 + T cells, ( E ) CD8 + T cells, and ( F ) Tregs (a. normal saline, b. NPC-AEAA-SLN, c. BAE-SLN, d. ABS, and e. NPC-ABS. Mean ± SD, n = 3. ** p < 0.01, *** p < 0.001. Blue color represented positive cells; the magnification was 10×).

Article Snippet: FITC Plus Anti-Mouse CD31 was obtained from Proteintech Group, Inc. (Chicago, IL, USA).

Techniques: Activation Assay, Confocal Microscopy, Saline, Labeling, Expressing